16s rrna gene 454  (Pyrosequencing Inc)

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: The gut microbiome: A line of defense against tuberculosis development

doi: 10.3389/fcimb.2023.1149679

Figure Lengend Snippet: Studies investigating the alteration of gut microbiome in pulmonary tuberculosis patients or intestinal tuberculosis patients without antibiotics comparing with the healthy controls.

Article Snippet: Stool samples from new PTB patients (n=83) , Stool samples from healthy controls (n=31) , decreased alpha-deversity , Firmicutes , Actinobacteria , Clostridales , Bifidobacteriales , Bifidobacteriaceae , Lachnospiraceae , Ruminococcaceae , Marinifilaceae , Eggerhellaceae , Barnesiellaceae , Blautia , Roseburia, Bifidobacterium , undifined Ruminococcaceae , Fusicatenibacter , Romboutsia decreased in patients; , 16S rRNA gene amplicon (454) pyrosequencing , ( ) .

Techniques: Sequencing, Amplification, Illumina Sequencing, Shotgun Sequencing

Journal: Cancers

Article Title: Microbiota Alterations in Precancerous Colon Lesions: A Systematic Review

doi: 10.3390/cancers13123061

Figure Lengend Snippet: Details of studies ( n = 19) included in the review. CRA: colorectal adenoma; CRC: colorectal carcinoma.

Article Snippet: Sanapareddy et al. , 2012 , 16S rRNA gene 454 titanium pyrosequencing , Abundance of a specific taxon was calculated by the delta–delta threshold cycle (DDCt) method. , 71 (33 subjects with adenomas and 38 subjects without adenomas (controls) , Mucosal biopsies , USA.

Techniques: Sequencing, Real-time Polymerase Chain Reaction, Produced, Mass Spectrometry, Bacteria, Control, Amplification, Positive Control, Transformation Assay, Construct, Plasmid Preparation